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Release of arachidonic acid via Ca 2+ increase stimulated by pyrophosphonucleotides and bradykinin in mammary tumour cells
Author(s) -
Enomoto Kohichi,
Furuya Kishio,
Yamagishi Shunichi,
Oka Takami,
Maeno Takashi
Publication year - 1995
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290130409
Subject(s) - arachidonic acid , bradykinin , phospholipase a2 , extracellular , intracellular , liberation , cytosol , phospholipase c , calmodulin , phospholipase , chemistry , biochemistry , phospholipase a , medicine , endocrinology , biology , enzyme , receptor , in vitro
The relationship between the increase of intracellular Ca 2+ and the release of arachidonic acid by bradykinin and pyrophosphonucleotides was studied in cultured mammary tumour cells, MMT060562. Bradykinin, ATP, UTP and UDP induced an increase of intracellular Ca 2+ and the release of arachidonic acid from phospholipids into the extracellular fluid. Release of arachidonic acid was also induced by the application of the Ca 2+ ionophore, A23187. Liberation of arachidonic acid by bradykinin and ATP was reduced by mepacrine, a blocker of phospholipase A 2 and W‐7, a calmodulin antagonist. It is suggested that the increase in cytosolic Ca 2+ ‐induced release of arachidonic acid occurs through activation of calmodulin‐dependent phospholipase A 2 .