Release of arachidonic acid via Ca 2+  increase stimulated by pyrophosphonucleotides and bradykinin in mammary tumour cells | Zendy

Enomoto Kohichi | Zendy; Furuya Kishio | Zendy; Yamagishi Shunichi | Zendy; Oka Takami | Zendy; Maeno Takashi | Zendy

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Release of arachidonic acid via Ca 2+ increase stimulated by pyrophosphonucleotides and bradykinin in mammary tumour cells

Author(s) -

Enomoto Kohichi,

Furuya Kishio,

Yamagishi Shunichi,

Oka Takami,

Maeno Takashi

Publication year - 1995

Publication title -

cell biochemistry and function

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.933

H-Index - 61

eISSN - 1099-0844

pISSN - 0263-6484

DOI - 10.1002/cbf.290130409

Subject(s) - arachidonic acid , bradykinin , phospholipase a2 , extracellular , intracellular , liberation , cytosol , phospholipase c , calmodulin , phospholipase , chemistry , biochemistry , phospholipase a , medicine , endocrinology , biology , enzyme , receptor , in vitro

The relationship between the increase of intracellular Ca 2+ and the release of arachidonic acid by bradykinin and pyrophosphonucleotides was studied in cultured mammary tumour cells, MMT060562. Bradykinin, ATP, UTP and UDP induced an increase of intracellular Ca 2+ and the release of arachidonic acid from phospholipids into the extracellular fluid. Release of arachidonic acid was also induced by the application of the Ca 2+ ionophore, A23187. Liberation of arachidonic acid by bradykinin and ATP was reduced by mepacrine, a blocker of phospholipase A 2 and W‐7, a calmodulin antagonist. It is suggested that the increase in cytosolic Ca 2+ ‐induced release of arachidonic acid occurs through activation of calmodulin‐dependent phospholipase A 2 .

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