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Mechanism of citrinin‐induced dysfunction of mitochondria. IV—Effect on Ca 2+ transport
Author(s) -
Chagas Generoso M.,
Oliveira Ma. Benigna M.,
Campello Annibal P.,
Kluppel Ma. Lucia W.
Publication year - 1995
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290130110
Subject(s) - citrinin , mitochondrion , kidney cortex , ruthenium red , hamster , kidney , chemistry , mitochondrial matrix , mycotoxin , biochemistry , biology , calcium , microbiology and biotechnology , endocrinology , cytosol , enzyme , food science , organic chemistry
The effect of citrinin on Ca 2+ transport was studied in isolated kidney cortex and liver mitochondria, and baby hamster kidney cultured cells. The mycotoxin significantly inhibited the activity of 2‐oxoglutarate and pyruvate dehydrogenases in both kidney cortex and liver mitochondria. Citrinin promoted a decrease in the velocity and in the total capacity of Ca 2+ uptake, in both mitochondria. Apparently, citrinin acts by a mechanism similar to ruthenium red. In intact cultured cells, citrinin also had a preferential effect on mitochondrial Ca 2+ fluxes. Citrinin promoted a marked decrease in the Ca 2+ level in the mitochondrial matrix, whereas that of the extramitochondiral fraction became less affected. All the observed effects were dependent on the citrinin concentration.