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Cell cycle effects of hypertonic stress on various human cells in culture
Author(s) -
Pellicciari C.,
Filippini C.,
De Grada L.,
Conti A. M. Fuhrman,
Romanini M. G. Manfredi
Publication year - 1995
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290130103
Subject(s) - cell cycle , bromodeoxyuridine , biology , flow cytometry , cell culture , tonicity , cell growth , cell , microbiology and biotechnology , cell type , cell division , biochemistry , genetics
Long‐term exposure to hypertonic (HT) culture media has been found to perturb the cell cycle and change gene expression in various animal cell types. A lower growth rate, with exit of cells from the cycling compartment has been observed previously in human transformed EUE. cells. The aim of this study was to investigate if the kinetic changes after long‐term HT stress, were typical of transformed cells or could be also found in primary cultures of normal cells. Human transformed cells from normal and neoplastic tissues, and normal human cells of epithelial and connective origin have been studied. After the incorporation of bromodeoxyuridine (BrdUrd), the frequency of S‐phase cells was estimated by dual‐parameter flow cytometry of DNA content versus BrdUrd immuno‐labelling; the total growth fraction was also estimated, after immunolabelling with an anti‐PCNA antibody. We also investigated, by polyacrylamide gel electrophoresis, changes in the amount of a 35 kDa protien band, which increased in EUE cells grown in an HT medium, and which may be directly involved in cell resistance to hypertonicity. Lower BrdUrd labelling indices and higher frequencies of cells in the G 0/1 range of DNA content were common features of all the cells in HT media, irrespective of their tissue of origin; other cycle phases may also be involved, depending on the cell type considered. The mechanisms by which cells cope with the HT environment could however, differ, since only some cell types showed an increase of the 35 kDa stress protein found originally in HT EUE cells.