Premium
Induction of differentiation by radiation and hyperthermia in neuroblastoma—glioma hybrid cells
Author(s) -
Sugihara Masaki,
Fujita Yasuhiko,
Enomoto KohIchi,
Maeno Takashi,
Ishida Tetsuya
Publication year - 1994
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290120209
Subject(s) - hyperthermia , intracellular , calcium , neuroblastoma , calcium in biology , acetylcholinesterase , chemistry , glioma , neurite , biophysics , cell culture , microbiology and biotechnology , biology , medicine , cancer research , biochemistry , in vitro , enzyme , genetics , organic chemistry
The effects of either radiation or hyperthermia on the differentiation potential of NG108‐15, a neuroblastoma‐glioma hybrid cell line, were studied. After radiation and hyperthermia, the outgrowth of neurites from NG108‐15 cells was potentiated, and polarizing current and voltage pulses induced a distinct action potential and a diphasic (inward following outward) current, respectively. An increase in the specific activity of acetylcholinesterase was also observed. In addition, both treatments induced an elevation of the concentration of intracellular calcium in some cells. The increase in intracellular calcium concentration caused by applying the calcium ionophore, A23187, induced differentiation. It is suggested that both the radiation‐ and the hyperthermia‐induced increases of electrical excitability and acetylcholinesterase activity may have originated from an increase in intracellular Ca 2+ concentration.