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The preparation and kinetic properties of multiple forms of chicken brain acetylcholinesterase
Author(s) -
AlJafari Abdulaziz A.,
Kamal M. A.
Publication year - 1994
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290120105
Subject(s) - acetylcholinesterase , kinetic energy , reaction rate constant , chemistry , solubility , turnover number , kinetics , enzyme , stereochemistry , biochemistry , physics , quantum mechanics
A method for preparing various forms of acetylcholinesterase (A ChE) from chicken brain has been developed and they have been characterized in terms of kinetic parameters such as K m , rate constant ( k ), turnover number ( k p ), specificity constant ( k sp ), V max and half‐life ( t 1/2 ). The solubility experiments show that, there are two major forms of A ChE i.e. water‐soluble and membrane‐bound A ChE (MBA ChE). The MBA ChE shows several subforms, and on the basis of percentage activity only three MBA ChE forms have been selected for complete characterization by various kinetic parameters. It was found that these three forms of MBA ChE demonstrate significant differences in their kinetic properties.