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Modifications of proteoglycans produced by human skin fibroblast cultures during replicative senescence
Author(s) -
Passi A.,
Albertini R.,
Bardoni A.,
Rindi S.,
Salvini R.,
Pallavicini G.,
De Luca G.
Publication year - 1993
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290110407
Subject(s) - senescence , fibroblast , microbiology and biotechnology , biology , cellular senescence , human skin , skin aging , genetics , cell culture , medicine , phenotype , dermatology , gene
Abstract The properties of proteoglycans (PGs) produced by normal human skin fibroblast were investigated with increasing passage. The increase of subculture number was associated with a constant increase in PG molecular size, which was particularly evident in cell layer extracts. In the cell layer, the ratio of DS‐PGs/HS‐PGs was markedly higher in early passage cultures. Moreover, the cell layer from young cells contained lower amounts of radioactivity incorporated into the most hydrophobic PG populations, suggesting that the PG core protein might also undergo significant modification with increasing subcultures. There was no significant difference in energy charge value between early and late passage cultures, whereas the NAD/NADH ratio was found to decrease markedly in senescent cells.