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Isolation and cloning by a polymerase chain reaction of a genomic DNA fragment of the human slow skeletal troponin (TNNT1) gene
Author(s) -
Novelli G.,
Gennarelli M.,
Sangiuolo F.,
D'Agruma L.,
Cicero S. Lo,
Melchionda S.,
Dallapiccola B.
Publication year - 1993
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290110306
Subject(s) - cloning (programming) , polymerase chain reaction , genomic dna , dna , fragment (logic) , gene , microbiology and biotechnology , primer (cosmetics) , molecular cloning , genetics , biology , chemistry , complementary dna , organic chemistry , computer science , programming language
The genomic 3′ structure of the gene coding for the human slow skeletal troponin T (TNNT1) gene, is reported. An intron of 912 nucleotides containing an Alu‐element has been identified and characterized. The complexity of the sequenced region suggests an alternative exon use. The present results may be valuable for further studies on the gene structure of TNNT1 and the related troponin gene family.