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Change of liver metabolism of 1,2‐dibromoethane during simultaneous treatment with carbon tetrachloride
Author(s) -
Chiarpotto E.,
Biasi F.,
Aragno M.,
Scavazza A.,
Danni O.,
Albano E.,
Poli G.
Publication year - 1993
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290110109
Subject(s) - carbon tetrachloride , metabolism , chemistry , carbon tetrachloride poisoning , carbon fibers , biochemistry , organic chemistry , materials science , composite number , composite material
The combination of carbon tetrachloride (CCl 4 ) and 1,2‐dibromoethane (DBE) in isolated rat hepatocytes led to a significant potentiation of both lipid peroxidation and of plasma membrane damage observed after a single treatment with CCl 4 . Such a synergistic effect appeared to be related to the CCl 4 ‐induced shift of DBE metabolism from the cytosolic conjugation with glutathione towards the microsomal transformation into toxic intermediates. In fact, CCl 4 significantly inactivated hepatocyte total GSH‐transferase, i.e. the DBE detoxification pathway. Furthermore, while the microsomal metabolism of CCl 4 was not affected by the simultaneous presence of DBE, the amount of DBE reactive metabolities covalently bound to hepatocyte protein was significantly enhanced in the presence of CCl 4 .