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Calciotropic hormones raise the chemically detectable [Pi] in UMR 106–06 osteoblast‐like cells
Author(s) -
Ahmado Abdulla,
Khouja Hamed I.,
Kemp Graham J.,
GuillandCumming Diane F.,
Russell R. Graham G.,
Bevington Alan
Publication year - 1993
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290110104
Subject(s) - pi , osteoblast , parathyroid hormone , intracellular , hormone , chemistry , medicine , endocrinology , bone cell , calcitonin , calcium , biochemistry , biology , in vitro
Uptake of orthophosphate (Pi) by osteoblast‐like cells is known to be stimulated by parathyroid hormone (PTH), but effects on intracellular [Pi] have not been investigated. Here we show in rat osteoblast‐like cells (UMR 106‐06) that PTH (10 −11 to 10 −7 M ) increases both 32 Pi uptake and cellular [Pi] by up to 50 per cent. 1,25 Dihydroxyvitamin D 3 (1,25D) (10 −12 to 10 −6 M ) and salmon calcitonin (CT) (10 −12 to 10 −6 g ml −1 ) also increased cellular [Pi] (by up to 60 per cent), but the percentage increases in total cellular 32 Pi uptake were smaller. The effects of 1,25D were transient (observable at 80 min and 6 h but not 24 h), and were also observed with 24,25 dihydroxy‐ and 25 hydroxyvitamin D 3 . Transient degradation of organic phosphorus pools to Pi might contribute to this increased [Pi]. These pools remain to be identified but were not shown to be phospholipids. Foetal bovine serum also affected cellular [Pi]. Care is therefore needed in distinguishing direct hormonal effects on cellular [Pi] from indirect effects arising from changes in the rate of cell growth.