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Modifications of adhesion properties and proteoglycan structure in rat embryo fibroblast cultures with increasing passages
Author(s) -
Pippia P.,
Formato M.,
Sciola L.,
Tilloca G.,
Bardoni A.,
Pallavicini G.,
Passi A.,
Rindi S.,
Salvini R.,
De Luca G.
Publication year - 1992
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290100312
Subject(s) - subculture (biology) , extracellular matrix , adhesion , fibroblast , cell adhesion , embryo , cell , microbiology and biotechnology , cell culture , proteoglycan , cell adhesion molecule , cell growth , chemistry , biology , biochemistry , genetics , organic chemistry
Adhesion properties of rat embryo fibroblast cultures and proteoglycans (PGs) produced both in the growth medium and in the cell layer were investigated with increasing passages. Both cell–cell and cell–substrate adhesion increased with increasing subculture number. Cell adhesion properties were improved by cell treatment with chondroitinase ABC. The increase in subculture number was coupled with a constant increase of PG molecular size, which was particularly evident in cell layer extracts. The ratio HS–PGs/DS–PGs increased with increasing passages. PG modifications are likely to represent evidence of changes in extracellular matrix organization and could play a role in the increase of cell adhesion properties.