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Added ATP influences some responses of rat synaptosomes to glutamate
Author(s) -
Galzigna L.,
Bianchi M.,
Rizzo V.,
Battistin T.,
Scarpa M.,
Rigo A.
Publication year - 1992
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290100206
Subject(s) - glutamate receptor , chemistry , synaptosome , biochemistry , biophysics , neuroscience , biology , membrane , receptor
ATP added externally to rat synaptosomes activated uptake of both Ca 2+ and glutamate which was partially accounted for by the uptake phenomena of synaptic vescles and mitochondria, as shown by using specific inhibitors of the latter. Increasing concentrations of glutamate stimulated Ca 2+ entry linearly, as shown by using 45 Ca or a Ca‐specific electrode. The processes of glutamate and Ca 2+ uptake shared some common features and their ATP‐dependence may be correlated with an ouabain‐insensitive synaptosomal ectonucleotidase activity measured by a 31 P‐NMR or a luminometric technique. The ATP hydrolysis catalysed by the synaptosomes was activated by both Ca 2+ and glutamate. The present synaptosomal activities may represent a model for studying the modulatory effects of ATP on the glutamatergic neurotransmission.