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Differentiation of human peripheral blood monocytes to macrophages is associated with changes in the cellular respiratory burst activity
Author(s) -
Johansson Agneta,
Dahlgren Claes
Publication year - 1992
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290100204
Subject(s) - respiratory burst , ionomycin , macrophage , monocyte , chemistry , chemiluminescence , intracellular , phagocytosis , peroxidase , luminol , reactive oxygen species , immunology , phagocyte , microbiology and biotechnology , biochemistry , biology , enzyme , in vitro , organic chemistry
When phagocytic leukocytes, e.g. neutrophils, monocytes and macrophages, interact with soluble or particulate stimuli, the cells respond with an increased production of reactive oxygen metabolites. This production can be measured with the luminol‐amplified chemiluminescence (CL) technique. In the present study, the CL reaction induced in monocyte‐derived macrophages was investigated and compared to the responses of neutrophils and monocytes. In systems without additives the CL response of macrophages to soluble stimuli (FMLP, PMA and ionomycin) was very low. Addition of a peroxidase (HRP) to the reaction mixtures resulted in a pronounced increase in CL activity. The cellular CL response in macrophages is thus limited by the amount of peroxidase available. The macrophage response differs qualitatively from the responses of neutrophils and monocytes, in that the intracellular phase of the response is missing.