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Reduced neutrophil superoxide generation and plasma lipoperoxide levels in pigs fed with cod liver oil
Author(s) -
Guarnieri Carlo,
YouZhou Li,
Roth Elizabeth,
Biagi Pierluigi,
Ussia Giovanni,
Baccarini Elisabetta,
Farruggia Francesco,
Anderson Gary L.,
Barker John H.,
Galletti Giovanni
Publication year - 1991
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290090405
Subject(s) - superoxide , thiobarbituric acid , chemistry , medicine , endocrinology , reactive oxygen species , neutrophile , biochemistry , biology , oxidative stress , lipid peroxidation , enzyme , in vitro
Circulating neutrophils, isolated from pigs fed for 8 weeks with a diet supplemented with CLO, had an accentuated n‐3 fatty acid incorporation into the plasma membrane, as evidenced by an approximately four‐fold greater n‐3/n‐6 ratio as compared with the control diet group. Moreover, the neutrophils of the CLO fed pigs produced less superoxide when stimulated with PMA or f‐MLP, as well as showing a more prolonged latency period before O 2 − ‐generation. In the plasma of pigs fed with CLO there were higher levels of thiobarbituric reactive material and lipofuscin, while the content of GSSG was similar in both dietary groups. The results of this study indicate that dietary supplementation with CLO reduces the activation of circulating neutrophils and favours the presence in the plasma of lipoperoxides.