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In vivo and in vitro evidence concerning the role of lipid peroxidation in the mechanism of hepatocyte death due to carbon tetrachloride
Author(s) -
Biasi Fiorella,
Albano Emanuele,
Chiarpotto Elena,
Corongiu Francesco P.,
Pronzato Maria A.,
Marinari Umberto M.,
Parola Maurizio,
Dianzani Mario U.,
Poli Giuseppe
Publication year - 1991
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290090208
Subject(s) - carbon tetrachloride , lipid peroxidation , hepatocyte , in vivo , vitamin e , ccl4 , chemistry , in vitro , tocopherol , pharmacology , incubation , biochemistry , medicine , antioxidant , biology , microbiology and biotechnology , organic chemistry
Isolated rat hepatocytes exposed to CCl 4 showed a stimulated formation of malonaldehyde after only 30–60 min incubation. Conversely, the onset of hepatocyte death was a relatively late event, being significant only after 2–3 h of treatment. A cause–effect relationship between the two phenomena has been demonstrated by using hepatocytes isolated from rats pretreated with alpha‐tocopherol. Comparable results were obtained in vivo where supplementation with alpha‐tocopherol 15 h before CCl 4 dosing induced a partial or complete protection against the drug's necrogenic effect, depending on the concentration of the haloalkane used. Moreover, the vitamin supplementation prevented the CCl 4 ‐induced increase of liver total calcium content, probably by blocking alterations in the liver cell plasma membranes due to lipid peroxidation.