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A 33 kDa protein band in enhanced during long‐term adaptation of EUE cells to a hypertonic medium
Author(s) -
Giuliani Attilia,
Ferraretto Anita,
Conti Anna Maria Fuhrman,
De Grada Laura,
Fraschini Annunzia,
Pellicciari Carlo,
Romanini Maria Gabriella Manfredi
Publication year - 1991
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290090204
Subject(s) - tonicity , cytosol , isotonic , microbiology and biotechnology , osmotic pressure , biophysics , biology , cytoskeleton , chemistry , biochemistry , cell , enzyme , medicine
A cell line derived from human embryonic epithelium (EUE cells) shows an enhanced expression of a 33 kDa protein when adapted to grow in a hypertonic medium containing 0·246 M NaCl (1·8 × the isotonic concentration). The maximum amount of this protein, followed by SDS‐PAGE electrophoresis, was found after 4 days of adaptation; thereafter, the protein band remained fairly constant up to 30 days. When the cells were transferred back to a medium containing 0·137 M NaCl (isotonic medium), the protein pattern reverted to that of control cells. This protein is mainly localized in the cytosol, although a small part is associated with the 150 000 g pellet and needs detergents to be extracted. The molecular weight and the cellular location suggest a possible analogy with the so‐called amphitropic proteins, that are known to interact with both the epidermal growth factor receptor and hydrophobic structures, such as the membrane phospholipids and the cytoskeletal components.