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Studies of the proliferation and differentiation of immature myeloid cells in vitro : 4: Preculture proto‐oncogene expression and the behaviour of myeloid leukemia cells in vitro
Author(s) -
Yin Mo Ying,
Gao Xue Zhi,
Wang Zique,
Preisler Harvey D.
Publication year - 1991
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290090107
Subject(s) - in vitro , myeloid leukemia , in vivo , myeloid , leukemia , cellular differentiation , biology , differentiation therapy , cancer research , cell culture , microbiology and biotechnology , immunology , biochemistry , genetics , acute promyelocytic leukemia , gene , retinoic acid
Studies were conducted to determine the relationship between the pretherapy characteristics of leukemia cells and their behaviour during culture in vitro . Leukemia cells which proliferated well in vitro also proliferated well in vivo . Cells which manifested myeloid or monocytic differentiation in vivo tended to manifest differentiation along these lines in vitro . Cells which manifested high levels of expression of c‐fms, c‐fes, or triose phosphate isomerase prior to culture were likely to differentiate in vitro , with high levels of c‐fes expression being related to myeloid maturation. These observations suggest that differentiation at the molecular level prior to culture is a requisite for leukemia cell differentiation in vitro . The same may be true for differentiation in vivo under the influence of exogenously administered agents such as cytotoxic chemotherapy or recombinant growth factors.