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Effects of vanadate on 6‐phosphofructo 2‐kinase activity and fructose 2,6‐bisphosphate levels in cultured rat hepatocytes
Author(s) -
Miralpeix Montserrat,
Katz Norbert R.,
Bartrons Ramon
Publication year - 1990
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290080409
Subject(s) - fructose 2,6 bisphosphate , vanadate , cycloheximide , phosphofructokinase 2 , fructose , biochemistry , enzyme , chemistry , metabolite , biology , glycolysis , protein biosynthesis , phosphofructokinase
The presence of vanadate in primary cultures of rat hepatocytes produced a significant increase in the concentration of fructose 2,6‐bisphosphate and in the activity of 6‐phosphofructo 2‐kinase. Compared with insulin, vanadate had a more potent action on the metabolite increase, but a similar effect on the 6‐phosphofructo 2‐kinase activity. Both the insulin‐ and the vanadate‐dependent enhancements of 6‐phosphofructo 2‐kinase were inhibited by cycloheximide which specificially blocks protein synthesis on the translational level, suggesting that the increase of the enzyme activity was due to induction rather than to a change in the catalytic activity.