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Carboxylesterase activities during the establishment of the procambium and procortex in embryos of pisum sativum L.
Author(s) -
Carmignac D. F.,
Gahan P. B.,
Loukopoulou Z.
Publication year - 1990
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290080408
Subject(s) - carboxylesterase , germination , radicle , embryo , sativum , pisum , chemistry , biology , enzyme , biochemistry , botany , microbiology and biotechnology
A cytochemical and electrophoretic analysis of alpha‐naphthyl and naphthol AS‐D acetate esterases from the radicles of pea seeds germinated for 24, 36 and 48 h permitted the detection of the loss of activity from the procortex with time after germination, but a stability of this activity in the prostele. Inhibitor studies showed the procambial enzyme as a single band of carboxylesterase activity the remainder being acetylesterase activity. The procambial carboxylesterase which is present in the more mature root apex corresponds to that of the 48‐h embryo (separating at R f 0·74).