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Balancing of energy‐consuming processes of rat hepatocytes
Author(s) -
Schneider W.,
Siems W.,
Grune T.
Publication year - 1990
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290080407
Subject(s) - computer science , energy (signal processing) , energy metabolism , biochemical engineering , chemistry , computational biology , microbiology and biotechnology , biological system , biology , physics , engineering , endocrinology , quantum mechanics
A method for the quantification of energy consuming processes described by Siems et al. 6 for reticulocytes and by Müller et al. 10 for ascites tumour cells was applied to balance the ATP‐consumption of isolated rat hepatocytes. On the basis of decreased coupled respiration rates following the specific inhibition of energy‐requiring reactions, the energy demands of protein turnover, nucleic acid synthesis, Na + /K + ‐ATPase and Ca 2+ ‐transport of hepatocytes in different incubation media were assessed. These processes together with urea synthesis account for about 60 per cent of the total energy consumption in a glucose and amino acid‐enriched Eagle/Borsook medium. The metabolic flux rates of total ATP‐consumption and ATP‐consumption of single energy‐requiring processes in hepatocytes are compared with those in reticulocytes and different tumour cell types.