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The activation of phosphatidylinositol‐specific phospholipase C by insulin in mammary epithelial cells of lactating mouse
Author(s) -
Yoshimoto Akio,
Nakanishi Keiko,
Anzai Tadashi,
Komine Senichi
Publication year - 1990
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290080306
Subject(s) - lactation , endoplasmic reticulum , phosphatidylinositol , golgi apparatus , prolactin , mammary gland , medicine , endocrinology , phospholipase c , organelle , insulin , biology , phospholipase d , phospholipase , inositol , inositol trisphosphate , chemistry , microbiology and biotechnology , biochemistry , hormone , enzyme , pregnancy , signal transduction , receptor , genetics , cancer , breast cancer
We have previously demonstrated in vitro that, in the endoplasmic reticulum and Golgi apparatus of mammary epithelial cells of lactating and pregnant mice, inositol 1,4,5‐trisphosphate releases Ca 2+ that has been stored in these organelles. In this study, we examined whether insulin and prolactin, essential for the growth of mammary gland and for lactation, influenced the activity of phosphatidylinositol‐specific phospholipase C in mammary cells. In the plasma membrane fraction of mammary epithelial cells of the DDY mouse strain 5 days after the start of lactation after the first pregnancy, and with phosphatidylinostol as substrate, it was shown that the activity of phospholipase C was enhanced by about four times in the presence of insulin compared with the control. Such enhancement was not found in the membrane fraction treated with prolactin.