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Suppressive effect of adrenalectomy on growth of L1210 leukemic cells in ascites
Author(s) -
Kimura Kotohiko,
Sakamoto Yukiya
Publication year - 1990
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290080203
Subject(s) - ascites , adrenalectomy , dexamethasone , peritoneal cavity , endocrinology , medicine , ehrlich ascites , inoculation , bilateral adrenalectomy , chemistry , tumor cells , pathology , cancer research , surgery
This study was designed to evaluate the effect of adrenalectomy on growth of L1210 leukemic cells in ascites of BDF 1 mice. Varying doses of 1·5 × 10 4 , 5·0 × 10 5 , and 1·5 × 10 6 viable tumour cells were inoculated intraperitoneally into groups of either adrenalectomized or sham‐operated mice. At days 4 to 7 after the inoculation, adrenalectomized mice inoculated with 1·5 × 10 4 or 5·0 × 10 5 tumour cells had a smaller number of tumour cells in ascites than sham‐operated controls. However, after inoculation of 1·5 × 10 6 cells, no significant differences were found at days 2 to 4 between adrenalectomized and sham‐operated mice. The growth retardation by adrenalectomy was not observed in adrenalectomized mice supplemented with 4 or 6 μg dexamethasone per day per mouse. It suggested that the ablation of glucocorticoids was at least partially responsible for the growth retardation observed in adrenalectomized mice. Cell kinetic analysis revealed that the difference in a potential doubling time could not explain these results. Tumour retention in the peritoneal cavity was measured using [ 125 I]‐iododeoxyuridine‐labelled tumour cells as a tracer. At days 4 to 6 after inoculation of 5·0 × 10 5 labelled cells, radioactivity in the peritoneal cavity in adrenalectomized mice was about 70 per cent of that in sham‐operated mice. This ratio was almost equivalent to the ratio of the number of cells in ascites of adrenalectomized mice to that of sham‐operated ones. Consequently, growth retardation observed in adrenalectomized mice resulted from an increase in tumour cell migration and/or in tumour cell death, but not from an increase in doubling time.