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Lack of inhibition of vasopressin‐stimulated Na + K + ATPase by atrial natriuretic factor in the rat renal medullary thick ascending limb of Henle's loop
Author(s) -
Charlton Judith A.,
Baylis Peter H.
Publication year - 1990
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290080105
Subject(s) - endocrinology , medicine , vasopressin , loop of henle , nephron , peptide hormone , arginine , kidney , diuretic , chemistry , stimulation , hormone , biology , amino acid , biochemistry
The anti‐diuretic hormone, arginine vasopressin (AVP) stimulates the activity of Na + K + ATPase in the rat renal medullary thick ascending limb of Henle's loop (mTAL). Atrial natriuretic factor (ANF) has been suggested to exert a tubular effect on the mammalian nephron, perhaps in part, by interacting with other hormones. In the present study, we investigated the effect of rat ANF with and without AVP upon mTAL Na + K + ATPase activity using cytochemical methods. ANF alone failed to inhibit or stimulate Na + K + ATPase activity in mTAL at any of the concentrations tested (10 nmol–0·1 pmol l −1 ). Unlike the rat hypothalamic digitalis‐like factor, ANF (10 nmol–10 fmol l −1 ) did not inhibit Na + K + ATPase activity after stimulation with AVP (l fmol l −1 ) for either 4 or 10 min. The results suggest that ANF does not exert an effect on mTAL, either alone or in conjunction with AVP.