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Evidence for a differential distribution of reducing equivalents from NADPH in cartilage
Author(s) -
Nahir A. M.,
Boussidan F.,
Shomart D.
Publication year - 1989
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290070409
Subject(s) - dehydrogenase , papain , cartilage , chemistry , biochemistry , respiration , glucose 6 phosphate dehydrogenase , equivalent , enzyme , biosynthesis , matrix (chemical analysis) , medicine , endocrinology , biology , anatomy , chromatography
It has been suggested that the NADPH, generated from the activity of glucose 6‐phosphate dehydrogenase, may be utilized by two different routes, namely either for biosynthetic purposes (type 2) or for microsomal respiration (type 1). This concept has been tested in the rabbit ear model in which an injection of papain into the rabbit causes loss of proteoglycans of the auricular matrix followed by its restoration over the following eight days. It is shown that whereas the type 1 pathway was either unaltered or diminished, the doubling of the glucose 6‐phosphate dehydrogenase activity was related solely to the type 2 pathway.