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The mechanism of placental alkaline phosphatase induction in vitro
Author(s) -
Nozawa Shiro,
Narisawa Sonoko,
Iizuka Rihachi,
Fukasawa Toshio,
Kohji Takehiko,
Nakane Paul K.,
Hirano Kazuyuki,
Millan Jose L.
Publication year - 1989
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290070312
Subject(s) - placental alkaline phosphatase , alkaline phosphatase , northern blot , western blot , microbiology and biotechnology , blot , in situ hybridization , biology , immunoassay , phosphatase , southern blot , enzyme , cell culture , messenger rna , biochemistry , immunology , gene , antibody , genetics
The mechanism of placental alkaline phosphatase (PLAP) induction by prednisolone in a uterine cervical epidermoid cancer cell line SKG‐IIIa was investigated in vitro by enzyme‐cytochemistry, enzyme immunoassay, Northern and Southern blot analysis, and in situ hybridization. Enzyme‐cytochemical alkaline phosphatase (ALP) staining and immunoassay revealed increased levels of PLAP (heat‐stable ALP) in prednisolone‐treated cells. Northern blot analysis and in situ hybridization showed increased amounts of PLAP mRNA. Southern blot analysis indicated that PLAP was not a product of an amplified or rearranged gene. These findings suggest that the induction of PLAP mRNA in SKG‐IIIa cells by prednisolone in turn increased the levels of PLAP.