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Effect of sodium arsentie on the induction and turnover of ornithine decarboxylase activity in erythroleukemia cells
Author(s) -
Flamigni Flavio,
Marmiroli Sandra,
Caldarera Claudio M.,
Guarnieri Carlo
Publication year - 1989
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290070310
Subject(s) - ornithine decarboxylase , chemistry , enzyme inducer , pharmacology , biochemistry , endocrinology , medicine , biology , enzyme
Sodium arsenite proved effective in preventing the induction of ornithine decarboxylase (ODC) activity elicited by dilution of Friend erythroleukemia cells in fresh medium. A 50 per cent inhibition was produced at approximately 1 μ M arsenite and complete inhibition was obtained at concentrations above 10 μ M . However, addition of arsenite 5 h after cell dilution, i.e. when ODC was already induced, appeared to stabilize the enzyme. The half‐life of ODC activity, measured after cycloheximide treatment, increased almost six‐fold after addition of sodium arsenite. Agents known to provoke oxidative alteration of the thiol‐redox status in cells, also caused a similar effect on the induction and stability of ODC.