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Methotrexate: Studies on cellular metabolism. III.‐Effect on the transplasma‐membrane redox activity and on ferricyanide‐induced proton extrusion by HeLa cells
Author(s) -
Oliveira Ma. Benigna M.,
Campello Annibal P.,
Klüppel Ma. Lúcia W.
Publication year - 1989
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290070209
Subject(s) - ferricyanide , hela , intracellular , chemistry , electron transport chain , redox , extrusion , metabolism , methotrexate , proton , biophysics , membrane , biochemistry , inorganic chemistry , cell , biology , materials science , physics , quantum mechanics , immunology , metallurgy
The effect of methotrexate (MTX) on transplasma‐membrane electron transport and ferricyanide‐induced proton extrusion by HeLa cells was studied. Both systems were inhibited by MTX. It is suggested that inhibition of electron transport and proton extrusion caused by MTX could be associated with other metabolic alterations such as response to the increase in NADH levels and decrease in intracellular pH.