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Clofibrate feeding increases cytoplasmic but not mitochondrial malic enzyme activity in rat kidney cortex
Author(s) -
Żelewski Mateusz,
Świerczyṅski Julian
Publication year - 1989
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290070204
Subject(s) - clofibrate , malic enzyme , enzyme , cytosol , mitochondrion , endocrinology , kidney , medicine , enzyme assay , citrate synthase , malic acid , malate dehydrogenase , specific activity , biochemistry , biology , chemistry , dehydrogenase , citric acid
Administration of clofibrate for 21 days to rats increased the malic enzyme activity in the kidney cortex by about 80 per cent. This effect seems to be specific since the drug did not alter significantly the activity either of lactate dehydrogenase, citrate synthase or total mitochondrial protein content in this organ. The increase in activity of malic enzyme in the 13 000 g supernatant (extramitochondrial) fraction in rats treated with the drug was about 80 per cent, whereas in the pellet (mitochondrial fraction) it was about 40 per cent. The specific activity of malic enzyme in the kidney cortex cytosol from clofibrate‐treated rats was about twice that in controls. In contrast clofibrate treatment did not affect its specific activity in isolated mitochondria. Calculations showed that 0·57 and 0·53 μmoles min −1 g −1 wet tissue of mitochondrial malic enzyme was obtained in control and clofibrate‐treated rats respectively. Thus, clofibrate feeding increases the amount of cytoplasmic but not mitochondrial malic enzyme activity.