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Some effects of fructose‐1,6‐diphosphate on rat myocardial tissue related to a membrane‐stabilizing action
Author(s) -
Galzigna L.,
Rizzoli V.,
Bianchi M.,
Rigobello M. P.,
Scuri R.
Publication year - 1989
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290070203
Subject(s) - extracellular , intracellular , chemistry , biophysics , stimulation , membrane , cell membrane , cell , biochemistry , contraction (grammar) , fructose , membrane permeability , biology , endocrinology
This study aims at elucidating the mechanism of action of extracellular fructose‐1,6‐diphosphate (FDP). FDP is able to inhibit Ca ++ entry into the myocardial tissue with an IC 50 value of 11·5 m M and in addition, it is bound by rat heart slices, the binding being activated by Zn and conditions of chemical hypoxia induced by KCN and iodoacetate. The overall effect of extracellular FDP includes an increase of frequency and amplitude of contraction of perfused heart at concentration below 1 m M , and, in general, a stimulation of the oxygen consumption of the tissue. The antihaemolytic effect of FDP suggests its action as a membrane stabilizer. The effects of extracellular FDP on the myocardial cell can be interpreted both on the basis of a limited permeability of the cell membrane to it and as a purely extracellular effect transduced through the cell membrane with a final response consisting of an increase in the intracellular FDP.