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Glycolysis and glutaminolysis in perifused ehrlich ascites tumour cells
Author(s) -
Segura J. A.,
Medina M. A.,
Alonso F. J.,
SanchezJimenez F.,
De Castro I. Núñez
Publication year - 1989
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290070103
Subject(s) - glutamine , glutaminolysis , metabolism , glycolysis , carbohydrate metabolism , biochemistry , azaserine , chemistry , medicine , glutaminase , glutamate receptor , endocrinology , biology , amino acid , receptor
A perifusion system was designed in order to study glucose and glutamine metabolism by freshly harvested Ehrlich ascites tumour cells in steady state conditions. Cells were perifused in the presence of 5 m M glucose, 0·5 m M glutamine or 5m M glucose and 0·5 m M glutamine. The results in steady state reveal that both substrates glucose and glutamine are continuously wasted by tumour cells, excreting two moles of lactate per mol of glucose and one mol of glutamate and ammonia per mol of glutamine consumed into the medium. Glutamine consumption in the presence of glucose was higher than with glutamine alone.