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Stimulatory effect of 1,25‐dihydroxyvitamin D3 on the glucose‐6‐phosphate dehydrogenase activity in the MCF‐7 human breast cancer cell line
Author(s) -
Noun Aïcha,
Garabedian Michèle,
Monet JeanDominique
Publication year - 1989
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290070102
Subject(s) - mcf 7 , pentose phosphate pathway , glucose 6 phosphate dehydrogenase , endocrinology , dehydrogenase , medicine , human breast , enzyme , chemistry , cell culture , breast cancer , hexose , receptor , enzyme assay , specific activity , calcitriol , biochemistry , biology , cancer , vitamin d and neurology , glycolysis , genetics
Human breast cancer cell lines have been shown to possess high affinity receptors for 1,25‐dihydroxyvitamin D3 (1,25(OH)2D3) on the activity of glucose‐6‐phosphate dehydrogenase (G6PD) in cells of a human breast cancer cell line MCF‐7. G6PD, an enzyme which controls the hexose monophosphate shunt, is elevated and sensitive to 17β‐estradiol in breast tumors. G6PD activity was stimulated by 1,25(OH)2D3 in a dose‐dependent manner at very low concentrations of steroid (10 −10 –10 −12 M ). 1,25(OH)2D3 increased maximum velocity without modifying the affinity constant of the enzyme for glucose‐6‐phosphate.