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Influence of long term treatment with 3‐methylcholanthrene or carbaryl on mixed‐function oxidase activity in 3T3 fibroblasts: Studies by microspectrofluorimetry on single cells
Author(s) -
Lahmy Sylvie,
Salmon JeanMarie,
Viallet Pierre
Publication year - 1988
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290060410
Subject(s) - pyrene , chemistry , methylcholanthrene , population , carbaryl , fibroblast , cell , carcinogen , biophysics , microbiology and biotechnology , biochemistry , biology , in vitro , organic chemistry , demography , sociology , pesticide , agronomy
Using benzo(a)pyrene (BaP) as a probe for aryl hydrocarbon hydroxylase (AHH) activity, differences in mixed‐function oxidase (MFO) activity were observed using microspectrofluorimetry in single living cells during long term treatment with 3‐methylcholanthrene (3‐MC) or carbaryl. Although these two compounds differ in chemical structure, similar effects were observed in 3T3 cell populations. The results suggest that the two compounds activate the same enzymatic system and that individual cells of a supposed homogeneous cell population are not equally sensitive to xenobiotics, i.e. subpopulations were observed which have differences in AHH activity.