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Inhibition of monocyte luminol‐dependent chemiluminescence by tetrahydrobiopterin, and the free radical oxidation of tetrahydrobiopterin, dihydrobiopterin and dihydroneopterin
Author(s) -
Heales Simon J. R.,
Blair John A.,
Meinschad Christian,
Ziegler Irmgard
Publication year - 1988
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290060307
Subject(s) - tetrahydrobiopterin , chemiluminescence , chemistry , luminol , biopterin , monooxygenase , biochemistry , cofactor , photochemistry , enzyme , organic chemistry , cytochrome p450
Luminol‐dependent chemiluminescence of normal human monocytes activated by zymosan is demonstrated to be inhibited by tetrahydrobiopterin in a concentration‐dependent manner. The reduced pterins tetrahydrobiopterin, dihydrobiopterin, and dihydroneopterin are all shown to be readily oxidized by the hydroxyl radical. The susceptibility of reduced pterins to free radical attack may explain the inhibition of chemiluminescence observed and an additional role of reduced pterins as free radical scavengers in tissues is considered.