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Changes in ribonucleoprotein particle and chromatin organization induced by liposomes in isolated nuclei
Author(s) -
Maraldi N. M.,
Galanzi A.,
Caramelli E.,
Billi A. M.,
Ognibene A.,
Rizzoli R.,
Capitani S.
Publication year - 1988
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290060304
Subject(s) - chromatin , ribonucleoprotein , liposome , ribonucleoprotein particle , biophysics , rna , cell nucleus , chemistry , microbiology and biotechnology , nucleus , biochemistry , dna , biology , gene
Nuclei isolated from rat liver, incubated in the presence of liposomes of different phospholipids, undergo typical modifications: chromatin dispersion and reduction of the interchromatin granules in nuclei incubated with negatively charged liposomes and increase of the chromatin density and of the number and size of the interchromatin granules in nuclei incubated with neutral liposomes. The possibility that the observed modifications are caused by an impairment of the transport and translocation of ribonucleoproteins belonging to the inner nuclear matrix, is suggested by the results obtained by radiotracer techniques on the release of RNA from liposome‐incubated nuclei.