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Adenylate cyclase in membrane fractions of RIN‐A2‐cells: Studies with forskolin, NaF, GppNHp and NEM
Author(s) -
Trier J. K.,
Kühn M.,
Ammon H. P. T.
Publication year - 1988
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290060208
Subject(s) - forskolin , adenylate kinase , cyclase , membrane , chemistry , biochemistry , enzyme , biology , microbiology and biotechnology , in vitro
In crude membrane fractions of rat pancreatic islets and of RIN‐A2‐cells, forskolin and NaF stimulated adenylate cyclase activity. Basal and stimulated enzyme activity was approximately 3 to 6 fold higher in membranes of RIN‐A2‐cells than in membranes of islet cells. In RIN‐A2‐cells GppNHp and NEM inhibited forskolin‐stimulated cyclase system of RIN‐A2‐cells contains inhibitory and stimulatory N‐proteins and that there are critical thiols related to N i , N s and/or the catalytic unit. Thus, membrane fractions of RIN‐A2‐cells may be an appropriate model for studies on the adenylate cyclase system of insulin‐producing cells.