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Effects of insulin and dexamethasone on adenine nucleotide levels in cultured hepatocytes from adult rat
Author(s) -
Gallo G.,
Mazzei M.,
Voci A.,
Fugassa E.
Publication year - 1988
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290060204
Subject(s) - dexamethasone , energy charge , insulin , nucleotide , hormone , medicine , endocrinology , hepatocyte , biology , adenine nucleotide , chemistry , biochemistry , in vitro , enzyme , adenylate kinase , gene
Insulin and dexamethasone, usually added to culture media, play an important role in maintaining the survival of functional hepatocytes. Adenine nucleotide concentrations and energy charge values of cultured hepatocytes were determined to investigate the relationship between the beneficial effects of these hormones and the energy status of the cells. The results indicate that insulin and dexamethasone are essential in maintaining the metabolic competence of cultured hepatocytes and that this correlates with the absolute concentration of ATP rather than with the energy charge.