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Tightly‐bound non‐histone proteins in different nucleosome‐like subpopulations from pig kidney chromatin
Author(s) -
Caiafa Paola,
Tomassetti Antonella,
Mastrantonio Stefania,
Reale Anna,
Spinelli Maurizio,
Strom Roberto
Publication year - 1988
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290060107
Subject(s) - chromatin , nucleosome , histone , biology , heterochromatin , dna , microbiology and biotechnology , non histone protein , biochemistry , biophysics
By differential sucrose gradient centrifugation of pig kidney chromatin in the presence or absence of Na‐EDTA and under varying ionic strength conditions, three nucleosome‐like subpopulations with different buoyant densities can be obtained. These particles, on the basis of their histones and HMG protein pattern, of the 5‐methylcytosine level of their DNA and of the RNA polymerase activity associated with them, can be considered as originating from chromatin fractions differently involved in gene expression. Two‐dimensional electrophoresis of the tightly‐bound non‐histone proteins shows a distinct pattern for each subpopulation, such protein components being notably present in restricted numbers but in high amounts in the subpopulation which was apparently derived from condensed heterochromatin.