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Initial adhesion of murine fibroblasts to collagen and fibronectin occurs by two mechanisms
Author(s) -
Bauvois Brigitte,
Roth Stephen
Publication year - 1987
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290050407
Subject(s) - fibronectin , adhesion , cell adhesion , sodium azide , chemistry , secretion , divalent , lysis , intracellular , antiserum , microbiology and biotechnology , extracellular matrix , extracellular , in vitro , cell adhesion molecule , biophysics , biochemistry , biology , immunology , antibody , organic chemistry
The adhesion of Balb/c 3T12 cells to fibronectin (FN) and to denatured (DC) or native (NC) collagen is differentially sensitive to divalent cations and to sodium azide. Short‐time adhesion (10 min) to FN requires either Mg 2+ or Mn 2+ , whereas only Mn 2+ stimulates attachment to DC and NC. Azide treatment only slightly affects adhesion of cells to FN, but strongly inhibits cell attachment to DC and NC. Attachment to any of these substrata is unaffected by monensin and by treatment of the cells with an intracellular fraction, making unlikely the possibility that molecules released by secretion or cell lysis participate in the adhesive process. Soluble collagen inhibits the adhesion of cells to DC and NC, but does not affect adhesion to FN. Finally, rabbit antiserum against collagen binding proteins inhibits cell attachment to NC and DC; the cells, however, attach normally to FN in presence of this antiserum. Taken together, our results support the view that 3T12 cells attach directly to native or denatured collagens and that FN is not required for this process.