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The preparative isolation of endosome fractions: A review
Author(s) -
Mullock Barbara M.,
Hinton Richard H.,
Peppard Jane V.,
Slot Jan W.,
Luzio J. Paul
Publication year - 1987
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290050402
Subject(s) - endosome , transcytosis , ultracentrifuge , chemistry , cell fractionation , size exclusion chromatography , intracellular , biochemistry , compartment (ship) , membrane , receptor , microbiology and biotechnology , isolation (microbiology) , electrophoresis , chromatography , endocytosis , biology , enzyme , bioinformatics , oceanography , geology
The endosome is an intracellular, acidic, membrane‐bound, subcellular compartment to which endocytosed ligands, receptors and plasma membrane proteins are conveyed before sorting and delivery to destinations elsewhere in the cell. The preparative isolation of elements of this compartment has been achieved successfully using various appropriate combinations of density gradient ultracentrifugation, electrophoretic, gel filtration and immunoaffinity techniques. These methods for isolating endosome fractions are reviewed together with the difficulties of establishing markers for such fractions. The isolation of an endosome fraction from the pathway of polymeric IgA transcytosis in rat liver is discussed to exemplify successful isolation procedures and appropriate subcellular markers.