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Synergism between cytosolic and mitochondrial oxidation: Its possible relevance to the metabolism of vitamin D 3 in the kidney
Author(s) -
Bachelet Maria,
Lair Micheline,
Thomas Michäel,
Monet Dominique,
Ulmann André,
Bader Cyrille
Publication year - 1986
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290040309
Subject(s) - cytosol , cytoplasm , mitochondrion , oxidative phosphorylation , biochemistry , kidney , chemistry , metabolism , cytochemistry , substrate (aquarium) , enzyme , function (biology) , microbiology and biotechnology , biology , endocrinology , ecology
Using frozen liver sections and quantitative cytochemistry it has been established that when cells are allowed to oxidize a cytoplasmic and a mitochondrial substrate simultaneously the resulting oxidative activity is markedly higher than the sum of the oxidation of each substrate measured separately. In the present study this type of synergistic interaction has been confirmed in the kidney, particularly in cells of the pars recta. Our results support the evidence of the influence of cytoplasmic NADPH on the intramitochondrial oxidative process and it is suggested that, in cells of the pars recta, cytosolic NADPH may be involved in intramitochondrial mixed function oxidases such as 1α‐hydroxylase: these results could further elucidate the mechanism responsible for the production of the hormonal form of vitamine D 3 .