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Aggregation states of cyclic nucleotide phosphodiesterase of murine thymocytes
Author(s) -
Sobolev A. S.,
Rybalkin S. D.
Publication year - 1986
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290040307
Subject(s) - phosphodiesterase , pde10a , cyclic nucleotide , cyclic nucleotide phosphodiesterase , enzyme , phosphodiesterase 3 , chemistry , nucleotide , size exclusion chromatography , biochemistry , centrifugation , gene
In murine thymocytes cyclic nucleotide phosphodiesterase is represented by cAMP‐ and cGMP‐specific forms. cAMP and cGMP phosphodiesterase activities showed anomalous kinetic behaviour indicative of ‘low’ and ‘high’ affinity enzyme forms. Sucrose density gradient centrifugation resolved only ‘low’ affinity forms of cAMP and cGMP phosphodiesterases. Gel filtration on Ultragel Aca 34 column showed that cAMP and cGMP phosphodiesterases are probably oligomeric enzymes. Storage of enzyme preparation at 4°C for 24–48 h led to a decrease of higher molecular weight form and enhancement of cAMP and cGMP phosphodiesterase activities.