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Formation of activated oxygen in the hypoxic rat liver
Author(s) -
Räder Lutz,
Siems Werner,
Müller Marianne,
Gerber Gerhard
Publication year - 1985
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290030408
Subject(s) - oxygen , hypoxia (environmental) , chemistry , microbiology and biotechnology , biochemistry , biology , organic chemistry
The biliary GSSG efflux rate of normoxic perfused rat liver was 1·5 ± 0·2 nmol/min/g liver wet weight. The GSSG efflux rate as indicator for the flux through the glutathine peroxidase and, therefore, for an oxidative loading incvreased with the extent of hypoxia. 2·6 ± 0·5 nmol/min/g were released from the severely hypoxic liver. The hydroxyl radical scavenger formate as well as the xanthine oxidase inhibitor allopurinol reduced the efflux rate of GSSG. GSH was released from the perfused liver at a rate of 15·5 nmol/min/g which was nearly unchanged in severe hypoxia. The high rate of glucose liberation from the hypoxic liver declined to almost that of the normoxic organ in the presence of formate. There is an ‘oxidative stress’ during hypoxic liver perfusion which probably originates from increased generation of activated oxygen species in the degradation of purine nucleotides.