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Studies on rat liver mitochondria. 6. The effect of contaminating particles in mitochondria stored at 0–4°C
Author(s) -
Higuti Ilma Hiroko,
Murata Lilia Yuko,
Stencel Mario,
Campello Annibal De Paiva,
Nascimento Aguinaldo José Do
Publication year - 1985
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290030407
Subject(s) - mitochondrion , monoamine oxidase , microsome , chemistry , submitochondrial particle , succinate dehydrogenase , biochemistry , enzyme
Rat liver mitochondria were stored at 0–4°C for several days using an appropriate medium and energy source. The elimination of the majority of microsomes and lysosomes, that normally contaminate isolated mitochondria, had a positive effect in preservation of respiratory control, P:O ratio, and monoamine oxidase activity during long term storage.