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The relationship between tissue preparation and function; methods for the study of control of aldosterone secretion: A review
Author(s) -
Vinson G. P.,
Hinson J. P.,
Raven P. W.
Publication year - 1985
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290030402
Subject(s) - aldosterone , in vivo , zona glomerulosa , corticosterone , secretion , in vitro , endocrinology , steroid , steroid 11 beta hydroxylase , medicine , biology , adrenal gland , chemistry , biochemistry , angiotensin ii , hormone , microbiology and biotechnology , blood pressure
The study of the control of aldosterone synthesis and secretion by the rat adrenal gland has over the past thirty years involved the application of many different in vivo and in vitro techniques. In this review the relationship between the data that each of these methods has produced is compared. There are striking differences in overall steroid production rates, and in the qualitative nature of the steroid profile which the various methods produce. In particular, aldosterone is secreted at higher rates in vivo , and when whole tissue preparations are used in vitro , than in incubations of isolated glomerulosa cells. In addition, while corticosterone is a major product of glomerulosa tissue in vitro , the available evidence suggests that it is not a major glomerulosa product in vivo .