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Role of transferrin in metal uptake by human lymphoblasts in vitro
Author(s) -
PlanasBohne Felicitas,
Taylor David M.,
Duffield John R.
Publication year - 1985
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290030309
Subject(s) - internalization , transferrin , endocytosis , chemistry , in vitro , kinetics , transferrin receptor , lymphoblast , biophysics , metal , biochemistry , binding site , receptor , cell culture , biology , physics , quantum mechanics , genetics , organic chemistry
The uptake and binding of 59 Fe, 67 Ga and 239 Pu complexed with citrate of transferrin (Tf) and of 125 I‐labelled Fe–Tf by human lymphoblasts (WI‐L2 cells) have been studied. Uptake kinetics of 59 Fe–Tf and [ 125 I]‐Tf point to internalization by receptor mediated endocytosis. 67 Ga binding and uptake is always less. This may be explained by a lower affinity of Ga‐complexes for the cell surface. Factors which influence Fe uptake have a similar effect on Ga. 239 Pu uptake and binding, however, are different, especially in that Tf does not stimulate 239 Pu uptake and may actually decrease it.