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Morphological changes in CHO and VERO cells treated with T‐2 mycotoxin. Correlation with inhibition of protein synthesis
Author(s) -
Trusal Lynn R.
Publication year - 1985
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290030308
Subject(s) - chinese hamster ovary cell , vero cell , protein biosynthesis , cycloheximide , bleb (medicine) , puromycin , biology , in vitro , microbiology and biotechnology , biochemistry , receptor , trabeculectomy , neuroscience , glaucoma
Exposure of Chinese hamster ovary and African green monkey kidney cells to T‐2 mycotoxin resulted in several morphological changes which were related to inhibition of protein synthesis, the basic in vitro mechanism of action of the toxin. These changes, which occurred in both cell types, included disassociation of polysomes and mitochondrial cristae alterations. In addition, CHO cells displayed membrane bleb formations similar to those found in CHO cells after exposure to established inhibitors of protein synthesis, puromycin and anisomycin. Blebs could be either a result of protein synthesis inhibition or a non‐specific early pathological response. Bleb formations were not observed in VERO cells under any experimental condition.