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The interaction between gluconeogenic metabolism and accumulation of phosphate by chick kidney tubule cells
Author(s) -
Grahn Michael F.,
Parveen Riffat,
Butterworth Peter J.
Publication year - 1985
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290030306
Subject(s) - metabolism , tubule , gluconeogenesis , phosphate , kidney , proximal tubule , endocrinology , chemistry , medicine , biology , microbiology and biotechnology , biochemistry
Pyruvate promotes both phosphate uptake and glucose synthesis by isolated chick kidney proximal tubule cells. 3‐Mercaptopicolinate inhibits both glucose synthesis and the promoted phosphate accumulation to the same extent. Glycerol also stimulates glucose synthesis, but does not affect phosphate accumulation. Oxygen utilization by the tissue is slightly stimulated by glycerol and pyruvate, but the enhancement of uptake by pyruvate is unlikely to result from raised cellular oxidative phosphorylation. The action of pyruvate is not a direct effect on the phosphate transporter, or on the transport of phosphate across the basolateral membrane, but entails an obligatory flux to triose phosphate.