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Human bone cell cultures: A new model for studying the mechanism of action of calcitonin
Author(s) -
Fanó G.,
Maurizi M.,
VentiDonti G.,
Paludetti G.,
Donti E.,
Della Torre G.
Publication year - 1985
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290030304
Subject(s) - calcitonin , adenylate kinase , cyclase , endocrinology , medicine , receptor , mechanism of action , bone cell , incubation , chemistry , calcitonin receptor , hormone , cell , cell culture , biology , biochemistry , in vitro , neuropeptide , calcitonin gene related peptide , genetics
An investigation on cell cultures obtained from temporal human bone fragments showed that they provide a suitable model for studying the mechanism involved in calcitonin action on bone cells. Furthermore they demonstrated: (1) a transitory increase in 45 Ca uptake that returned to control values ten minutes after the hormone was added; (2) a relation between 45 Ca uptake and increased cAMP concentrations when these were measured at the same time intervals; (3) a reproduction of the salmon calcitonin (sCT) effect after incubation of the cultures with either db‐cAMP or db‐cGMP and (4) inhibition of 45 Ca uptake and parallel decrease in cAMP levels with propanol. These results suggest that in human bone cell cultures, sCT acts as a temporary promoter of 45 Ca uptake, probably by activating an adenylate‐cyclase system through a β‐receptor.