Partial immunochemical identity between (Na +  + K + )‐atpase and a membrane component extractable with chloroform: Methanol | Zendy

Addis John S. | Zendy; Tenbarge Karen M. | Zendy; Barrnett Russell J. | Zendy

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Partial immunochemical identity between (Na + + K + )‐atpase and a membrane component extractable with chloroform: Methanol

Author(s) -

Addis John S.,

Tenbarge Karen M.,

Barrnett Russell J.

Publication year - 1985

Publication title -

cell biochemistry and function

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.933

H-Index - 61

eISSN - 1099-0844

pISSN - 0263-6484

DOI - 10.1002/cbf.290030209

Subject(s) - immunoelectrophoresis , antiserum , chloroform , fraction (chemistry) , chemistry , membrane , antigen , atpase , chromatography , methanol , biochemistry , microbiology and biotechnology , enzyme , biology , organic chemistry , immunology

An IgG fraction prepared from an antiserum against a holoenzyme preparation of (Na + + K + )‐ATPase precipitated a single antigen when samples of holoenzyme were subjected to crossed immunoelectrophoresis but precipitated an additional, immunochemically‐related antigen when a plasma membrane‐enriched fraction was subjected to crossed immunoelectrophoresis under the same conditions. The immunochemically‐related antigen could be extracted from the plasma membrane fraction with CHCl 3 :CH 3 OH.

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