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Partial immunochemical identity between (Na + + K + )‐atpase and a membrane component extractable with chloroform: Methanol
Author(s) -
Addis John S.,
Tenbarge Karen M.,
Barrnett Russell J.
Publication year - 1985
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290030209
Subject(s) - immunoelectrophoresis , antiserum , chloroform , fraction (chemistry) , chemistry , membrane , antigen , atpase , chromatography , methanol , biochemistry , microbiology and biotechnology , enzyme , biology , organic chemistry , immunology
An IgG fraction prepared from an antiserum against a holoenzyme preparation of (Na + + K + )‐ATPase precipitated a single antigen when samples of holoenzyme were subjected to crossed immunoelectrophoresis but precipitated an additional, immunochemically‐related antigen when a plasma membrane‐enriched fraction was subjected to crossed immunoelectrophoresis under the same conditions. The immunochemically‐related antigen could be extracted from the plasma membrane fraction with CHCl 3 :CH 3 OH.