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A catabolin‐like factor from porcine kidney
Author(s) -
Monserrat J. Juan,
Dingle John T.
Publication year - 1985
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290030110
Subject(s) - medulla , kidney , cortex (anatomy) , chemistry , explant culture , cartilage , ammonium , endocrinology , medicine , biology , anatomy , biochemistry , in vitro , organic chemistry , neuroscience
Explants of pig kidney cortex and medulla release a catabolin‐like factor (CLF). The CLF of both kidney cortex and medulla can be precipitated with ammonium sulphate, mainly in the 60–95 per cent fraction. By gel chromatography the kidney CLF showed a major active fraction at a molecular weight of around 22 500. Whole glomeruli and dissociated glomerular cells in culture also released into the medium a CLF that could be bioassayed in live cartilage but displayed no effect on dead cartilage. The possible role of such a local hormone is discussed.