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Alpha‐Lactalbumin as a lysosomal enzyme‐releasing factor
Author(s) -
Komine Senichi,
Nakanishi Keiko,
Anzaio Tadashi,
Yoshimoto Akio
Publication year - 1985
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290030108
Subject(s) - lactalbumin , enzyme , alpha lactalbumin , involution (esoterism) , mammary gland , in vitro , chemistry , biochemistry , lysosome , biology , microbiology and biotechnology , consciousness , genetics , cancer , neuroscience , breast cancer
Abstract In the early stage of mammary gland involution, biochemically detectable lysosomal damage occurs. The mechanism(s) underlying this damage is not well understood. We found that α‐lactalbumin from mouse milk induced the release of enzymes from the lysosomes of mouse mammary epithelial cells in vitro , and this induction also occurred with bovine α‐lactalbumin. This enzyme release was accelerated by the addition of whey proteins with a molecular weight of 50 000 to 60 000. We also found that the lysosomal membrane of mammary epithelial cells had a strong affinity for α‐lactalbumin.